FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9531616
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC100980.10594216132920169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT509650.072.7923661
AGCAGTG574850.064.430432
CAGTGGT727400.050.7553184
GCAGTGG760150.048.7658963
AGTGGTA760450.048.585045
TGGTATC782000.047.1149067
GTGGTAT783300.047.0670556
GGTATCA794800.046.425958
GTATCAA811950.045.519169
CAACGCA788400.027.14974612-13
ATCAACG790500.027.06559410-11
AGAGTAC822650.025.6374518-19
ACGCAGA837650.025.59428614-15
TACATGG817100.025.41897822-23
AGTACAT823500.025.40957620-21
GCAGAGT843800.025.24552716-17
TCAACGC784100.023.6289812-13
ACGACGC3250.023.5555115
CATGGGA359700.023.31899624-25
CGCAGAG801300.023.14476416-17
Produced by FastQC (version 0.11.5)