FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9526001
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103160.10829308122054573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC101530.10658197495465305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT495250.072.459751
AGCAGTG556700.064.428022
CAGTGGT715450.050.209214
GCAGTGG747000.048.211313
AGTGGTA755200.047.5566835
TGGTATC770650.046.516197
GTGGTAT776200.046.2326626
GGTATCA784450.045.7370388
GTATCAA799150.044.9276589
CAACGCA776850.026.63177112-13
ATCAACG778200.026.58795410-11
AGAGTAC810400.025.1809418-19
ACGCAGA827300.025.03632214-15
AGTACAT812650.024.88816320-21
GCAGAGT827750.024.8614516-17
TACATGG807100.024.79706622-23
TCAACGC771650.023.20939812-13
CATGGGA350300.023.03553824-25
CGCAGAG788250.022.73538416-17
TATCAAC805250.022.35123610-11
Produced by FastQC (version 0.11.5)