FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10982466
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC119920.10919223423955969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT576350.072.98461
AGCAGTG653900.064.2896652
CAGTGGT836450.050.247214
GCAGTGG878850.047.928213
AGTGGTA880800.047.7092555
TGGTATC898050.046.656097
GTGGTAT902000.046.484156
GGTATCA917550.045.7945638
GTATCAA938850.044.7829939
CAACGCA911650.026.82561912-13
ATCAACG913600.026.74887810-11
AGAGTAC951250.025.33329818-19
ACGCAGA973450.025.18460514-15
AGTACAT953650.025.03787820-21
TACATGG944900.025.02837622-23
GCAGAGT977250.024.90940716-17
TCAACGC906500.023.26176812-13
CATGGGA407500.022.89085824-25
CGCAGAG926500.022.78228616-17
TATCAAC943150.022.3748510-11
Produced by FastQC (version 0.11.5)