FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10978356
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC119560.10890519491260804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT577000.072.722121
AGCAGTG651200.064.317432
CAGTGGT830600.050.3426554
GCAGTGG866250.048.410353
AGTGGTA872150.047.9901585
TGGTATC893400.046.7207767
GTGGTAT899050.046.455346
GGTATCA908650.046.0170828
GTATCAA930650.044.974979
CAACGCA907100.026.72570612-13
ATCAACG910000.026.65478310-11
ACGACGC3000.025.5211075
AGAGTAC948600.025.24409318-19
ACGCAGA964100.025.19645714-15
AGTACAT945850.025.09886420-21
TACATGG938550.025.02823322-23
GCAGAGT970600.024.87061316-17
TCAACGC903000.023.22741112-13
CATGGGA409600.022.93582324-25
CGCAGAG921950.022.75830716-17
Produced by FastQC (version 0.11.5)