FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8921143
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC93280.10456059274019036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT479050.072.22291
AGCAGTG534000.064.5898742
CAGTGGT680450.050.553744
GCAGTGG712000.048.4992833
AGTGGTA722700.047.661285
TGGTATC734050.046.835067
GTGGTAT735650.046.74216
GGTATCA749700.045.9420558
GTATCAA762100.045.2578629
CAACGCA741500.026.79022212-13
ATCAACG743350.026.72483810-11
AGAGTAC774450.025.3361918-19
ACGCAGA790900.025.2375714-15
AGTACAT774200.025.17292220-21
TACATGG766150.025.1447222-23
GCAGAGT791850.024.98173516-17
TCAACGC737500.023.4155612-13
CATGGGG305250.023.3531624-25
CGCAGAG753300.022.92581716-17
CATGGGA329700.022.67209624-25
Produced by FastQC (version 0.11.5)