FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8908534
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC94730.1063362389367319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT472750.072.471391
AGCAGTG532050.064.2191242
CAGTGGT675700.050.5777974
GCAGTGG713700.048.0185663
AGTGGTA715550.047.7707145
TGGTATC733350.046.545097
GTGGTAT736450.046.3620726
GGTATCA742850.045.9928978
GTATCAA758850.045.1068089
ACGACGC3100.031.4731775
CAACGCA738950.026.81981712-13
ATCAACG740500.026.79041510-11
AGAGTAC770500.025.47030318-19
ACGCAGA785850.025.2832314-15
AGTACAT770900.025.24283420-21
TACATGG762950.025.17429422-23
GCAGAGT788400.025.06923116-17
CGACGCT4500.023.5721726
TCAACGC734750.023.28340712-13
CATGGGA330500.023.208324-25
Produced by FastQC (version 0.11.5)