FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9034690
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC96370.10666663715080428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT472550.071.925921
AGCAGTG535650.063.301182
CAGTGGT680850.049.5825964
GCAGTGG716650.047.2877733
AGTGGTA716250.047.1774865
TGGTATC730600.046.101687
GTGGTAT734750.045.9136926
GGTATCA744850.045.272818
GTATCAA761350.044.37769
CAACGCA740000.026.67984612-13
ATCAACG740450.026.65653210-11
AGAGTAC772600.025.2073318-19
ACGCAGA786400.025.15847214-15
AGTACAT772200.024.9241320-21
GCAGAGT790050.024.8623216-17
TACATGG767600.024.77834122-23
CATGGGA334900.023.15152224-25
TCAACGC734950.023.06480612-13
CGCAGAG750700.022.60999716-17
CATGGGG300850.022.37370724-25
Produced by FastQC (version 0.11.5)