FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8965962
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC96320.10742851687303605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT472350.072.279951
AGCAGTG530900.064.123342
CAGTGGT674250.050.5152974
GCAGTGG708300.048.233223
AGTGGTA712350.047.8663945
TGGTATC722200.047.0690467
GTGGTAT728100.046.781066
GGTATCA734150.046.328698
GTATCAA750300.045.3793759
CAACGCA729250.026.91434912-13
ATCAACG731850.026.8229910-11
AGAGTAC759850.025.43630218-19
ACGCAGA776450.025.33301714-15
TACATGG754000.025.1743422-23
AGTACAT762200.025.13780420-21
GCAGAGT782450.024.95867516-17
TCAACGC725650.023.49926412-13
CATGGGA326450.023.24929424-25
CGCAGAG740050.023.03442416-17
CATGGGG296050.022.95166624-25
Produced by FastQC (version 0.11.5)