FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8842629
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC94250.10658594859062841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT482300.071.876451
AGCAGTG535500.064.6078342
CAGTGGT679550.050.8274654
AGTGGTA710950.048.6194085
GCAGTGG714200.048.4688953
TGGTATC730050.047.191797
GTGGTAT734050.047.0157246
GGTATCA742450.046.4881488
GTATCAA754350.045.7850889
CAACGCA734200.027.0050412-13
ATCAACG736800.026.91175810-11
ACGACGC2950.025.8913565
AGAGTAC767050.025.55959518-19
ACGCAGA780000.025.46705414-15
GCAGAGT783550.025.2611116-17
AGTACAT768550.025.2421220-21
TACATGG763550.025.20786522-23
CATGGGA330350.023.65642224-25
TCAACGC730350.023.63033512-13
CGCAGAG744200.023.21866416-17
Produced by FastQC (version 0.11.5)