FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8780555
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98540.11222525227619437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC95360.1086036133251258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT464850.072.63271
AGCAGTG525900.064.06692
CAGTGGT669050.050.2930224
GCAGTGG700750.048.1392823
AGTGGTA701300.048.011265
TGGTATC716300.046.849547
GTGGTAT721150.046.5920646
GGTATCA730650.046.0273258
GTATCAA744350.045.219799
ACGACGC3550.033.4637765
CAACGCA724050.027.01195112-13
ATCAACG727100.026.9267110-11
CGACGCT4500.026.4038726
AGAGTAC756600.025.54602418-19
ACGCAGA769950.025.46854214-15
AGTACAT753750.025.42882320-21
TACATGG747700.025.41191122-23
GCAGAGT772600.025.23329516-17
CACGACG4800.024.742624
TCAACGC719300.023.44228212-13
Produced by FastQC (version 0.11.5)