FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10096559
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC107660.10663038763998704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT536350.071.910881
AGCAGTG601750.064.044292
CAGTGGT761900.050.4465374
GCAGTGG804750.047.8946233
AGTGGTA806750.047.684155
TGGTATC820150.046.7217677
GTGGTAT825150.046.5449456
GGTATCA835250.045.8772668
GTATCAA853000.044.9799589
ACGACGC3550.031.0635265
CAACGCA827400.026.89828912-13
ATCAACG831350.026.79893910-11
AGAGTAC859750.025.56240818-19
ACGCAGA880500.025.34256214-15
AGTACAT863900.025.24904420-21
TACATGG856300.025.15854522-23
GCAGAGT883950.025.10601216-17
CATGGGA376550.023.5032924-25
TCAACGC824050.023.33684712-13
CGCAGAG842100.022.87369716-17
Produced by FastQC (version 0.11.5)