FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10084810
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111620.11068131179466942No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC105480.10459294721467235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT533900.072.589211
AGCAGTG601450.064.290532
CAGTGGT764400.050.412384
GCAGTGG801850.048.2288483
AGTGGTA804450.048.0093655
TGGTATC818100.047.1174437
GTGGTAT822650.046.93246
GGTATCA833100.046.294698
GTATCAA850400.045.4610679
ACGACGC4000.036.050925
CAACGCA826550.027.1197912-13
ATCAACG830850.027.02627610-11
CGACGCT5450.026.4655656
AGAGTAC861150.025.62321918-19
ACGCAGA879200.025.54012914-15
AGTACAT858800.025.46635220-21
TACATGG851100.025.42385122-23
GCAGAGT883200.025.2397416-17
CACGACG6350.024.706864
TCAACGC823100.023.53212712-13
Produced by FastQC (version 0.11.5)