FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10147217
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109570.10798034574405968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC107290.10573342424824461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT534000.071.67211
AGCAGTG598150.063.9719352
CAGTGGT758300.050.4183544
GCAGTGG796500.048.1131553
AGTGGTA797200.047.97855
TGGTATC817250.046.7327657
GTGGTAT824450.046.3492436
GGTATCA831350.045.9663738
GTATCAA853600.044.8238149
CAACGCA827450.026.73365812-13
ATCAACG830750.026.63596210-11
AGAGTAC861550.025.28655818-19
ACGCAGA882500.025.10765614-15
AGTACAT864600.025.03535720-21
TACATGG857600.024.92005322-23
GCAGAGT886900.024.899316-17
TCAACGC823300.023.26612-13
CATGGGA375500.023.17298924-25
CGCAGAG842850.022.79662716-17
ACGACGC4100.022.7660145
Produced by FastQC (version 0.11.5)