FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11679685
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC124050.10621005617874112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT610200.072.118851
AGCAGTG686000.064.099362
CAGTGGT878600.049.8985064
GCAGTGG917600.047.938493
AGTGGTA924900.047.4582445
TGGTATC952400.045.934977
GTGGTAT956350.045.7970736
GGTATCA967450.045.2483378
GTATCAA990650.044.2375839
ACGACGC4300.027.6130085
CAACGCA963400.026.34141512-13
ATCAACG966000.026.29559710-11
AGAGTAC1006350.024.92755518-19
ACGCAGA1023700.024.85770814-15
AGTACAT1003800.024.79065520-21
GCAGAGT1025050.024.68023916-17
TACATGG1000950.024.60501522-23
CATGGGA434400.022.97564324-25
TCAACGC956400.022.89622512-13
CGCAGAG976550.022.43516216-17
Produced by FastQC (version 0.11.5)