FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11624055
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC123350.10611615309803679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT619650.072.228641
AGCAGTG694200.064.324132
CAGTGGT885700.050.3512734
GCAGTGG924300.048.355953
AGTGGTA931850.047.91535
TGGTATC952700.046.7663887
GTGGTAT955750.046.603896
GGTATCA965700.046.2124988
GTATCAA983150.045.434059
ACGACGC3750.028.2764155
CAACGCA956950.026.95197312-13
ATCAACG961450.026.84410-11
AGAGTAC1001650.025.5107818-19
ACGCAGA1021200.025.31739214-15
TACATGG991550.025.24538222-23
AGTACAT1006250.025.20233520-21
GCAGAGT1024300.025.11130516-17
TCAACGC952100.023.47691712-13
CATGGGA437350.023.46731624-25
CGCAGAG973250.022.97615416-17
Produced by FastQC (version 0.11.5)