FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11809283
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC125810.10653483365586208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT618050.072.430321
AGCAGTG697400.064.0551152
CAGTGGT895250.049.8633354
GCAGTGG939100.047.6055563
AGTGGTA944550.047.3826565
TGGTATC964250.046.248677
GTGGTAT969600.046.05566
GGTATCA981750.045.4780048
GTATCAA1001800.044.6169979
CAACGCA973600.026.62985412-13
ATCAACG978550.026.5296410-11
AGAGTAC1015150.025.20588918-19
ACGCAGA1032250.025.2011414-15
AGTACAT1016600.025.00134720-21
TACATGG1009950.024.84237322-23
GCAGAGT1043350.024.7837116-17
ACGACGC4550.024.2411845
TCAACGC970250.023.08707612-13
CATGGGA434500.022.92605624-25
CGCAGAG987800.022.71164716-17
Produced by FastQC (version 0.11.5)