FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11709271
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC124380.10622352151555806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT613800.072.596291
AGCAGTG695300.063.9672432
CAGTGGT885700.050.073774
GCAGTGG927500.047.962593
AGTGGTA931450.047.656165
TGGTATC947850.046.702457
GTGGTAT951400.046.5896036
GGTATCA964200.045.9644858
GTATCAA985200.045.0249189
CAACGCA957650.026.97131312-13
ATCAACG961100.026.89411410-11
AGAGTAC1001050.025.47145818-19
ACGCAGA1019450.025.35993214-15
ACGACGC4350.025.3599225
AGTACAT1005000.025.2069320-21
TACATGG995500.025.14611622-23
GCAGAGT1025200.025.09617616-17
CATGGGA435200.023.56390224-25
TCAACGC951900.023.34209312-13
CGCAGAG972650.022.87297416-17
Produced by FastQC (version 0.11.5)