FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10640997
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC112920.1061178759847409No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112270.10550703096711708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT551050.072.4579541
AGCAGTG620350.064.164692
CAGTGGT794450.050.0744484
AGTGGTA832750.047.8183255
GCAGTGG835400.047.6907843
TGGTATC852800.046.5695237
GTGGTAT856850.046.3921626
GGTATCA865450.045.8703278
GTATCAA882200.045.0488439
CAACGCA860700.026.73394612-13
ATCAACG864050.026.65089410-11
AGAGTAC898050.025.33191718-19
ACGCAGA917550.025.13279214-15
TACATGG888300.025.07278822-23
AGTACAT898250.025.05115320-21
GCAGAGT922450.024.9187316-17
ACGACGC4300.023.6441635
TCAACGC856550.023.23267212-13
CATGGGA386950.023.0464924-25
CGCAGAG873850.022.83101316-17
Produced by FastQC (version 0.11.5)