FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10656338
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC113450.10646246393460869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT549750.072.1823961
AGCAGTG622450.063.5950362
CAGTGGT792450.049.98484
GCAGTGG834850.047.5877653
AGTGGTA833850.047.554125
TGGTATC849600.046.5590067
GTGGTAT852050.046.443936
GGTATCA863600.045.8424458
GTATCAA880750.044.9021159
CAACGCA854400.026.69059212-13
ATCAACG855650.026.6889610-11
ACGACGC4050.026.1508165
AGAGTAC896800.025.24810418-19
ACGCAGA908400.025.19213314-15
TACATGG878800.025.18277522-23
AGTACAT894050.025.10068120-21
GCAGAGT912800.025.00228116-17
TCAACGC849000.023.28396812-13
CATGGGA385000.022.92880424-25
CGCAGAG868600.022.85488116-17
Produced by FastQC (version 0.11.5)