FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10739750
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC115220.10728368909890826No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113830.10598943178379384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT565550.072.278721
AGCAGTG635400.064.188732
CAGTGGT804700.050.727374
GCAGTGG849200.048.1258963
AGTGGTA853250.047.881395
TGGTATC863800.047.100467
GTGGTAT870300.046.8445666
GGTATCA881500.046.1745878
GTATCAA899400.045.256949
CAACGCA870700.027.0342612-13
ATCAACG874450.026.95145410-11
AGAGTAC913500.025.57550618-19
ACGCAGA929400.025.37401414-15
AGTACAT911600.025.36443120-21
TACATGG907500.025.23914122-23
GCAGAGT934700.025.17533316-17
ACGACGC3750.024.86585
CATGGGA398800.023.49380124-25
TCAACGC866100.023.47511512-13
CGCAGAG886550.022.98861916-17
Produced by FastQC (version 0.11.5)