FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10644852
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC114450.10751676021423313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT555000.072.207841
AGCAGTG622650.064.257312
CAGTGGT790350.050.389574
GCAGTGG829750.048.2166143
AGTGGTA832750.047.854945
TGGTATC847850.046.8668757
GTGGTAT850800.046.748116
GGTATCA865000.045.9758578
GTATCAA885000.044.991029
CAACGCA857650.026.88194512-13
ATCAACG861350.026.79307410-11
AGAGTAC897650.025.34830718-19
ACGCAGA917150.025.1869514-15
TACATGG888550.025.14928622-23
AGTACAT897650.025.11776520-21
GCAGAGT917350.025.03099616-17
ACGACGC3400.024.9302165
TCAACGC853200.023.40013912-13
CATGGGA389250.023.20159124-25
CGCAGAG873600.022.90844216-17
Produced by FastQC (version 0.11.5)