FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10408493
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC114140.10966044748264711No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111830.10744110602754886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT549150.072.219571
AGCAGTG616450.064.212052
CAGTGGT786700.050.2979164
GCAGTGG824400.048.1055223
AGTGGTA829550.047.7471475
TGGTATC846400.046.6447947
GTGGTAT849150.046.5386436
GGTATCA862400.045.8193668
GTATCAA878800.044.9615029
CAACGCA851850.026.85314612-13
ATCAACG854900.026.77782410-11
AGAGTAC891050.025.40781618-19
ACGCAGA910050.025.23239314-15
AGTACAT890800.025.17692620-21
TACATGG882200.025.14708122-23
GCAGAGT913800.024.97955116-17
TCAACGC849050.023.28338212-13
CATGGGA384350.023.09119424-25
CGCAGAG867100.022.88525216-17
CATGGGG347750.022.60021424-25
Produced by FastQC (version 0.11.5)