FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10404189
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC110670.10637061668141554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104580.10051720513727692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT535300.072.222891
AGCAGTG605750.063.6864432
CAGTGGT774100.049.6474154
GCAGTGG816950.047.2143
AGTGGTA816350.047.129835
TGGTATC837350.045.77347
GTGGTAT840300.045.702816
GGTATCA852300.045.039038
GTATCAA866950.044.323859
CAACGCA843250.026.47675112-13
ATCAACG846700.026.376810-11
ACGACGC4050.026.153125
AGAGTAC883500.024.97775518-19
ACGCAGA900650.024.87264614-15
TACATGG874900.024.7089522-23
GCAGAGT901550.024.70349316-17
AGTACAT886250.024.70003120-21
CATGGGA384050.023.29526924-25
TCAACGC838100.022.9834512-13
CGCAGAG857100.022.54148916-17
Produced by FastQC (version 0.11.5)