FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10404953
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC112880.10848679470248448No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104530.10046177046643076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT546400.072.5044861
AGCAGTG612350.064.623382
CAGTGGT782200.050.413834
GCAGTGG817750.048.340763
AGTGGTA823500.047.8964735
TGGTATC838100.046.9955987
GTGGTAT843900.046.6977166
GGTATCA851400.046.2812968
GTATCAA872200.045.232149
CAACGCA845600.026.89720312-13
ATCAACG847750.026.81806410-11
AGAGTAC884100.025.36114718-19
ACGCAGA902500.025.24985514-15
GCAGAGT902000.025.13757916-17
AGTACAT882900.025.13384420-21
TACATGG874250.025.08444222-23
TCAACGC840800.023.50890512-13
CATGGGA377350.023.2106224-25
CGCAGAG858500.023.0548516-17
ACGACGC3150.022.871565
Produced by FastQC (version 0.11.5)