FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12042772
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC127810.10613005045682174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT631200.071.6292341
AGCAGTG704250.064.048032
CAGTGGT892000.050.4717034
GCAGTGG928150.048.6008723
AGTGGTA940750.047.9102825
TGGTATC956100.047.017487
GTGGTAT963800.046.7114076
GGTATCA974500.046.1898848
GTATCAA996200.045.227979
CAACGCA965650.026.99364912-13
ATCAACG969900.026.92942210-11
AGAGTAC1014750.025.60158718-19
ACGCAGA1026050.025.51917314-15
TACATGG997400.025.44292622-23
AGTACAT1012100.025.43286120-21
GCAGAGT1034350.025.23752616-17
ACGACGC3900.023.8979265
CATGGGA439150.023.60462824-25
TCAACGC960350.023.44175512-13
CGCAGAG980700.023.07816916-17
Produced by FastQC (version 0.11.5)