FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12039620
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC126220.10483719585834106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120680.10023572172543653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT616550.071.9885251
AGCAGTG691150.064.035292
CAGTGGT875150.050.3904044
GCAGTGG919250.048.1309173
AGTGGTA924400.047.7798545
TGGTATC940050.046.850327
GTGGTAT947050.046.5696076
GGTATCA958950.045.8998268
GTATCAA984750.044.7558259
ACGACGC3250.027.3809155
CAACGCA955050.026.71970712-13
ATCAACG960100.026.5682210-11
AGAGTAC998850.025.2435718-19
ACGCAGA1019400.025.11546914-15
AGTACAT998150.025.0217820-21
TACATGG990600.024.90808122-23
GCAGAGT1024200.024.84436616-17
TCAACGC949450.023.31436312-13
CATGGGA439600.023.03447724-25
CGCAGAG971050.022.91119216-17
Produced by FastQC (version 0.11.5)