FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13878782
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC148870.10726445591551188No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141980.10230004333233275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT718250.072.086041
AGCAGTG808200.063.9141242
CAGTGGT1035650.049.840914
GCAGTGG1080200.047.877523
AGTGGTA1092100.047.3506855
TGGTATC1118250.046.1145027
GTGGTAT1121900.046.0002446
GGTATCA1135350.045.4769138
GTATCAA1162550.044.4054039
CAACGCA1126200.026.72364812-13
ATCAACG1129950.026.63140710-11
AGAGTAC1175700.025.22746818-19
ACGCAGA1195400.025.2043514-15
AGTACAT1173500.025.0437120-21
TACATGG1166200.024.93921522-23
GCAGAGT1209600.024.73882516-17
CATGGGA505000.023.72901724-25
TCAACGC1119550.023.08938212-13
CGCAGAG1143450.022.6130916-17
TATCAAC1164550.022.23866310-11
Produced by FastQC (version 0.11.5)