FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13867426
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC148910.1073811390808936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT719400.072.4771041
AGCAGTG816350.063.678312
CAGTGGT1034000.050.2552834
GCAGTGG1084300.048.038333
AGTGGTA1089400.047.722955
TGGTATC1111150.046.680337
GTGGTAT1118200.046.3925936
GGTATCA1128250.045.996258
GTATCAA1153350.045.066849
ACGACGC5000.031.349535
CAACGCA1118850.026.96584512-13
ATCAACG1120900.026.92095610-11
AGAGTAC1167150.025.52741418-19
CGACGCT6000.025.42126
ACGCAGA1196950.025.25451914-15
AGTACAT1169100.025.24143620-21
TACATGG1161150.025.17783422-23
GCAGAGT1198600.025.04332416-17
TCAACGC1112250.023.38745112-13
CATGGGA506050.023.11713624-25
Produced by FastQC (version 0.11.5)