FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13848495
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC148800.10744849891630823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT719300.071.829141
AGCAGTG804750.063.991162
CAGTGGT1024500.050.1280754
GCAGTGG1068100.048.2184223
AGTGGTA1079850.047.6332325
TGGTATC1100450.046.5960587
GTGGTAT1105650.046.3947456
GGTATCA1119900.045.8271378
GTATCAA1146500.044.8727389
ACGACGC4950.029.1119735
CAACGCA1113900.026.62792812-13
ATCAACG1116050.026.57910-11
AGAGTAC1163800.025.2155218-19
ACGCAGA1189700.024.98816914-15
AGTACAT1166350.024.92426320-21
TACATGG1154300.024.88308722-23
GCAGAGT1191950.024.83869616-17
CATGGGA505150.023.46494924-25
TCAACGC1106150.023.2725912-13
CGCAGAG1130000.022.81590816-17
Produced by FastQC (version 0.11.5)