FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9586342
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN184950.19293073416325018No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC102690.1071211521558484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT496400.071.601451
AGCAGTG559250.063.4447332
CAGTGGT714950.049.6263774
GCAGTGG749500.047.4456863
AGTGGTA753050.047.1670845
TGGTATC765950.046.2810677
GTGGTAT770450.045.9942056
GGTATCA780650.045.426858
GTATCAA795600.044.605769
CAACGCA774600.026.72593712-13
ATCAACG774950.026.69720610-11
AGAGTAC808850.025.33885418-19
ACGCAGA824500.025.15851414-15
AGTACAT809000.025.13662720-21
TACATGG801700.025.1058322-23
GCAGAGT826800.025.02268816-17
CATGGGA348100.023.34322724-25
TCAACGC769450.023.08888212-13
CGCAGAG786800.022.63795316-17
CATGGGG319500.022.34680624-25
Produced by FastQC (version 0.11.5)