FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9354327
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN184430.19716009500202422No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC100840.10780037943937604No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98850.10567302169359699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT485150.072.5932851
AGCAGTG551900.063.8024752
CAGTGGT702050.050.064984
GCAGTGG738400.047.806573
AGTGGTA738900.047.6039355
TGGTATC759000.046.2399147
GTGGTAT763700.045.99286
GGTATCA769600.045.5851148
GTATCAA790900.044.3957639
ACGACGC4100.030.0477755
CGACGCT4600.026.7825746
CAACGCA764550.026.77207212-13
ATCAACG768150.026.67850510-11
AGAGTAC800550.025.22400918-19
ACGCAGA815700.025.1405714-15
AGTACAT800800.025.05454320-21
TACATGG796050.024.94044322-23
GCAGAGT819600.024.91818216-17
CATGGGA351500.023.1352324-25
TCAACGC761350.023.05869112-13
Produced by FastQC (version 0.11.5)