FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9360872
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN212820.2273506143444756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC101210.10812026913731969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT489250.072.947691
AGCAGTG557650.063.919692
CAGTGGT709300.050.150564
GCAGTGG746500.047.763963
AGTGGTA751300.047.4217645
TGGTATC762050.046.606297
GTGGTAT768900.046.1900866
GGTATCA776050.045.821238
GTATCAA790900.045.015339
ACGACGC4350.032.22625
CGACGCT4800.029.2068776
CAACGCA770600.027.0208812-13
ATCAACG772750.026.9518510-11
CACGACG5400.025.9593014
AGAGTAC807500.025.54291518-19
ACGCAGA821400.025.41244914-15
TACATGG800050.025.31360422-23
AGTACAT808550.025.29196220-21
GCAGAGT827650.025.131516-17
TCAACGC765900.023.27831312-13
Produced by FastQC (version 0.11.5)