FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9385413
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN131240.13983401689408875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC100760.10735808855721106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97750.1041509840856231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT492250.072.630911
AGCAGTG560200.063.7664572
CAGTGGT714950.049.8623964
GCAGTGG748150.047.788343
AGTGGTA756950.047.1750765
TGGTATC774900.045.934517
GTGGTAT777950.045.7983556
GGTATCA785450.045.399888
GTATCAA800950.044.5338139
CAACGCA780550.026.7003712-13
ATCAACG784950.026.59209410-11
AGAGTAC813350.025.32982418-19
TACATGG804300.025.17401322-23
ACGCAGA830650.025.16770614-15
AGTACAT812650.025.11126920-21
GCAGAGT834300.024.89017716-17
CATGGGA351850.023.2695624-25
TCAACGC776650.023.05371912-13
ACGACGC2800.022.7601595
CGCAGAG792300.022.65349416-17
Produced by FastQC (version 0.11.5)