FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9367628
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN180780.1929837521302084No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC102730.10966490129625131No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94760.10115687770692859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT490600.072.460961
AGCAGTG552600.064.196482
CAGTGGT704900.050.2708364
GCAGTGG739100.048.1500663
AGTGGTA741950.047.842475
TGGTATC758200.046.6805087
GTGGTAT763000.046.4030536
GGTATCA774150.045.791078
GTATCAA790350.044.854649
CAACGCA767800.026.73994812-13
ATCAACG769300.026.70911810-11
AGAGTAC803000.025.2738118-19
ACGCAGA816800.025.14337214-15
TACATGG796400.025.08104722-23
AGTACAT804450.025.07240920-21
GCAGAGT819050.025.02514316-17
ACGACGC3750.024.9248875
TCAACGC762450.023.21573412-13
CATGGGA346550.023.21498524-25
CGCAGAG780850.022.71446616-17
Produced by FastQC (version 0.11.5)