FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10819036
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN208670.19287300643051747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC117430.10854016938292839No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111710.10325319187402648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT558600.072.098871
AGCAGTG631950.063.63942
CAGTGGT806400.049.792574
GCAGTGG836800.048.0988043
AGTGGTA848000.047.4165765
TGGTATC861200.046.6078877
GTGGTAT866850.046.341596
GGTATCA876900.045.8591888
GTATCAA895450.044.9343579
ATCAACG869600.026.9670810-11
CAACGCA867800.026.9582912-13
AGAGTAC907850.025.46399918-19
ACGCAGA928050.025.29307214-15
AGTACAT907950.025.24119820-21
TACATGG902450.025.11776422-23
GCAGAGT933100.025.03245716-17
CATGGGA387750.023.51458424-25
TCAACGC862000.023.38828712-13
CGCAGAG882800.022.89898716-17
ACGACGC3950.022.5853925
Produced by FastQC (version 0.11.5)