FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10825330
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN250770.2316511367320904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC116420.10754406563125557No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109200.10087452299375631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT561350.072.3842241
AGCAGTG633150.064.062762
CAGTGGT805950.050.3094024
GCAGTGG839650.048.4008263
AGTGGTA844450.048.0918355
TGGTATC860700.047.0594647
GTGGTAT864350.046.937646
GGTATCA877300.046.204428
GTATCAA896800.045.1855439
ACGACGC3850.027.5845995
CAACGCA870450.026.9542712-13
ATCAACG873350.026.87749310-11
AGAGTAC910450.025.51905818-19
ACGCAGA930700.025.28234514-15
TACATGG902250.025.24745822-23
AGTACAT912400.025.22970620-21
GCAGAGT932450.025.11961216-17
TCAACGC865900.023.45498512-13
CATGGGA397950.023.45241724-25
CGCAGAG882900.023.03720916-17
Produced by FastQC (version 0.11.5)