FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10845467
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN149810.13813144238048947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC116400.10732594548487401No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111000.10234690677681282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT559100.072.7290951
AGCAGTG633150.064.025882
CAGTGGT810400.049.930224
GCAGTGG848600.047.8577423
AGTGGTA854050.047.4434285
TGGTATC873350.046.250117
GTGGTAT880300.045.9516876
GGTATCA893250.045.2925538
GTATCAA909100.044.499049
CAACGCA883400.026.53308512-13
ATCAACG886150.026.51161210-11
AGAGTAC928000.025.04621518-19
ACGCAGA941300.024.93510614-15
AGTACAT924200.024.92887320-21
TACATGG916150.024.8981622-23
GCAGAGT946350.024.6997416-17
TCAACGC878000.023.04223312-13
CATGGGA401600.022.96932824-25
CGCAGAG897800.022.55408916-17
CATGGGG358700.022.31600424-25
Produced by FastQC (version 0.11.5)