FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12489347
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN172290.13794956613824566No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC133010.10649876250535756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125300.10032550140531765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT642900.072.049131
AGCAGTG723200.063.8966142
CAGTGGT930800.049.5657544
GCAGTGG964950.047.874573
AGTGGTA975050.047.360875
TGGTATC993600.046.3576857
GTGGTAT1000350.046.099546
GGTATCA1009200.045.671678
GTATCAA1033400.044.6699
CAACGCA1005850.026.65354312-13
ATCAACG1008400.026.58470210-11
AGAGTAC1055700.025.12226918-19
ACGCAGA1071700.025.059514-15
AGTACAT1054200.024.9486820-21
TACATGG1043150.024.92409922-23
GCAGAGT1074150.024.8605716-17
TCAACGC999450.023.11032712-13
CATGGGA459400.022.80345724-25
CGCAGAG1022900.022.61439116-17
CATGGGG412950.022.38908624-25
Produced by FastQC (version 0.11.5)