FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8869301
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC93210.10509283651552698No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91560.10323248697952635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT465900.072.289581
AGCAGTG524950.064.067982
CAGTGGT668450.050.1067244
GCAGTGG701500.047.838373
AGTGGTA707550.047.3978355
TGGTATC716850.046.5963447
GTGGTAT722850.046.28456
GGTATCA729850.045.8146488
GTATCAA747900.044.8041889
ATCAACG726650.026.7418410-11
CAACGCA726900.026.73660312-13
AGAGTAC757450.025.37256618-19
ACGCAGA776300.025.09352914-15
TACATGG750150.025.0837822-23
AGTACAT761100.024.98127620-21
GCAGAGT775450.024.95388816-17
ACGACGC2850.023.7960285
TCAACGC721550.023.26711812-13
CATGGGA326400.023.26526324-25
CGCAGAG737250.022.81521216-17
Produced by FastQC (version 0.11.5)