FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9053319
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC95240.10519898834891381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT479350.071.9969251
AGCAGTG534100.064.560662
CAGTGGT682900.050.4810754
GCAGTGG717300.048.127543
AGTGGTA720450.047.9138375
TGGTATC736000.046.790217
GTGGTAT740800.046.5023966
GGTATCA748800.046.084768
GTATCAA762550.045.3113639
CAACGCA741000.026.87295312-13
ATCAACG744300.026.79321510-11
AGAGTAC772850.025.47716918-19
ACGCAGA789650.025.27961514-15
AGTACAT774250.025.18459520-21
TACATGG767350.025.17348922-23
GCAGAGT791600.025.07848716-17
TCAACGC736950.023.48732412-13
CATGGGA343700.023.2097124-25
CGCAGAG751550.023.06539516-17
CATGGGG298700.022.7071324-25
Produced by FastQC (version 0.11.5)