FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9064743
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC96000.10590482267395777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT485900.072.21671
AGCAGTG544050.064.269882
CAGTGGT688850.050.7799034
GCAGTGG721250.048.621923
AGTGGTA728100.048.018945
TGGTATC745600.046.855447
GTGGTAT748450.046.674966
GGTATCA759500.046.0164458
GTATCAA777250.045.0781829
CAACGCA754900.026.74481612-13
ATCAACG756750.026.7129210-11
AGAGTAC788800.025.2916318-19
ACGCAGA804000.025.19839714-15
AGTACAT789200.025.10061820-21
TACATGG781300.025.0792622-23
GCAGAGT806300.024.92846516-17
ACGACGC3650.024.3971675
TCAACGC751150.023.33710512-13
CATGGGA337750.023.1053424-25
CGCAGAG766600.022.90462316-17
Produced by FastQC (version 0.11.5)