FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9010862
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC96020.10656028246798141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT476450.072.210191
AGCAGTG534400.064.24132
CAGTGGT682700.050.266084
GCAGTGG719150.047.840263
AGTGGTA719300.047.6944245
TGGTATC733150.046.7081877
GTGGTAT738050.046.420296
GGTATCA745900.045.9815678
GTATCAA763100.045.0176479
CAACGCA740600.026.88266212-13
ATCAACG742850.026.81160510-11
ACGACGC2450.025.9533445
AGAGTAC770400.025.5444118-19
ACGCAGA788500.025.30743414-15
AGTACAT773100.025.300620-21
GCAGAGT792050.025.08145716-17
TACATGG769150.025.06407522-23
TCAACGC736500.023.3577412-13
CATGGGA331800.023.3549324-25
CGCAGAG752000.022.95384416-17
Produced by FastQC (version 0.11.5)