FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8688598
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC92620.10659947669347805No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91030.10476949215512099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT465100.072.4846951
AGCAGTG524500.064.309242
CAGTGGT664850.050.6710934
GCAGTGG692100.048.80043
AGTGGTA695650.048.4540025
TGGTATC710250.047.3285877
GTGGTAT715950.047.0152856
GGTATCA723300.046.564468
GTATCAA741250.045.4860239
CAACGCA719050.027.037812-13
ATCAACG720300.026.99517610-11
AGAGTAC750100.025.64169118-19
AGTACAT748300.025.47934320-21
ACGCAGA765300.025.47117414-15
TACATGG742800.025.33608822-23
GCAGAGT768900.025.20535516-17
TCAACGC714850.023.61315212-13
CATGGGA322350.023.45929524-25
CGCAGAG730200.023.15489816-17
TATCAAC744900.022.73683510-11
Produced by FastQC (version 0.11.5)