FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8854354
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC94670.10691914960707467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT475500.072.61751
AGCAGTG535800.064.4442
CAGTGGT679400.050.7773484
GCAGTGG712500.048.6121943
AGTGGTA711450.048.4393045
TGGTATC731300.046.9014857
GTGGTAT735750.046.737726
GGTATCA743600.046.1811568
GTATCAA757850.045.327589
CAACGCA735950.026.96234312-13
ATCAACG737800.026.89905410-11
AGAGTAC765650.025.66498818-19
ACGCAGA781000.025.45533814-15
AGTACAT769400.025.28334820-21
TACATGG762200.025.25524322-23
GCAGAGT785800.025.15020816-17
ACGACGC2750.024.6535115
TCAACGC731350.023.47587612-13
CGCAGAG748050.023.01447116-17
CATGGGG305100.022.83672524-25
Produced by FastQC (version 0.11.5)