FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8882379
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC94260.10612021846849813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91280.10276526142376946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT465500.072.0421751
AGCAGTG529850.063.1965522
CAGTGGT666650.050.1220474
GCAGTGG703150.047.6321143
AGTGGTA703150.047.5631265
TGGTATC718000.046.474427
GTGGTAT719500.046.3708536
GGTATCA731550.045.678398
GTATCAA745550.044.785889
ACGACGC3150.026.919745
CAACGCA726800.026.71826712-13
ATCAACG728000.026.63960810-11
AGAGTAC758700.025.25621618-19
ACGCAGA772700.025.18330214-15
TACATGG746400.025.1059622-23
AGTACAT757200.025.06765420-21
GCAGAGT775100.024.95974216-17
TCAACGC721700.023.2013312-13
CATGGGA325050.023.15833524-25
CGCAGAG736200.022.79841616-17
Produced by FastQC (version 0.11.5)