FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10214612
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC109560.10725811220240182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT543950.072.2778241
AGCAGTG609250.064.3102952
CAGTGGT776200.050.396564
GCAGTGG813050.048.2958833
AGTGGTA811850.048.2162745
TGGTATC829800.047.0064937
GTGGTAT835650.046.6864286
GGTATCA845450.046.2387168
GTATCAA868800.045.0865069
ACGACGC2500.027.1364865
CAACGCA840650.026.94269212-13
ATCAACG845800.026.7836910-11
AGAGTAC876900.025.56481418-19
ACGCAGA893500.025.3769414-15
AGTACAT881000.025.23025120-21
GCAGAGT897850.025.14860216-17
TACATGG874200.025.14672722-23
TCAACGC837150.023.43461212-13
CATGGGA377850.023.27538724-25
CGCAGAG854250.023.00512516-17
Produced by FastQC (version 0.11.5)