FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10164331
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC108500.10674583501855656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT540700.072.5098341
AGCAGTG604600.064.709612
CAGTGGT767700.050.895894
GCAGTGG803000.048.867453
AGTGGTA809750.048.3283045
TGGTATC828250.047.094367
GTGGTAT832000.046.9462476
GGTATCA843350.046.322668
GTATCAA858500.045.5114829
CAACGCA835150.026.82588212-13
ATCAACG838050.026.75332810-11
AGAGTAC871100.025.50340718-19
ACGCAGA887050.025.31562214-15
AGTACAT873350.025.18005220-21
TACATGG865750.025.12010422-23
ACGACGC3550.025.0726725
GCAGAGT893600.025.04037316-17
TCAACGC829750.023.54489712-13
CGCAGAG846600.023.13547916-17
CATGGGA381600.023.07162324-25
Produced by FastQC (version 0.11.5)