FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11544245
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC123060.10659856924380937No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115700.10022309817575771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT600950.072.502141
AGCAGTG677300.064.132512
CAGTGGT870400.049.862294
AGTGGTA912650.047.5559355
GCAGTGG915350.047.5263563
TGGTATC932750.046.3916857
GTGGTAT937500.046.1780366
GGTATCA949950.045.59918
GTATCAA970450.044.7765059
CAACGCA941400.026.7468812-13
ATCAACG945750.026.63945410-11
AGAGTAC980450.025.39689818-19
ACGCAGA999500.025.22856114-15
TACATGG969750.025.21488622-23
AGTACAT982150.025.10585220-21
GCAGAGT1002750.025.03438816-17
TCAACGC936400.023.22590412-13
CATGGGA420350.023.12674124-25
CGCAGAG955250.022.79325516-17
CATGGGG384200.022.63641424-25
Produced by FastQC (version 0.11.5)