FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11796890
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC123990.10510397231812792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT620350.072.03211
AGCAGTG697250.063.9258542
CAGTGGT892500.049.9608354
AGTGGTA935000.047.7332575
GCAGTGG935550.047.7123873
TGGTATC954400.046.5599947
GTGGTAT959750.046.3749736
GGTATCA974600.045.7054948
GTATCAA998200.044.7012949
CAACGCA961850.026.90403712-13
ATCAACG966900.026.78658110-11
AGAGTAC1008750.025.43752718-19
ACGCAGA1025350.025.27501914-15
AGTACAT1009300.025.23445120-21
TACATGG999550.025.16435422-23
GCAGAGT1028650.025.07656516-17
TCAACGC958000.023.26809912-13
CATGGGA432700.023.01034424-25
CGCAGAG977100.022.83240316-17
CATGGGG394500.022.79273624-25
Produced by FastQC (version 0.11.5)