FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11714753
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC126280.10779570000323524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT623550.072.478151
AGCAGTG703750.064.083792
CAGTGGT889250.050.56044
GCAGTGG934450.048.3033
AGTGGTA934950.048.1630025
TGGTATC960500.046.7403767
GTGGTAT964400.046.5971266
GGTATCA974400.046.106648
GTATCAA994150.045.264149
CAACGCA969300.026.97646712-13
ATCAACG971300.026.9573210-11
AGAGTAC1012850.025.47366718-19
ACGCAGA1032550.025.37723514-15
AGTACAT1014950.025.19753620-21
TACATGG1009100.025.0928222-23
GCAGAGT1038400.025.04795816-17
TCAACGC964000.023.41104912-13
CATGGGA439200.023.27322424-25
CGCAGAG986550.022.89562616-17
CATGGGG397600.022.62368824-25
Produced by FastQC (version 0.11.5)